Fig 3. Effect of simvastatin and mevalonate in secreted fibulin 2 expression in culture medium from human coronary artery SMCs.

Cells were preincubated for 2 hours with mevalonate (200 μM) and treated with simvastatin for 24 hours. Blot bands were normalized to actin and quantified with Quantity One Analysis Software version 4.6.2. A representative Western blot is shown. The results are shown as the mean with the standard error of the mean (SEM) for four independent experiments in 3A (1 with cell lot number 4C1284 and 3 with cell lot number 886619) and three independent experiments in 2B with cell lot number 886619. Comparisons were performed using ANOVA followed by Dunett post-test correction.