Table 1. Experimental Design—Vaccination ^a .

		Test Material
Group	Test Material (Formulation Concentration, μg/ mL)	Target Dosage	Route	Vaccination Schedule	Dose Volume	Number of Rabbits b
1	Control	None	None	None	None	5 / 5 = 10
2	Vehicle Control	(0 μg)	IM	Day 0 and 28	0.5 mL	5 / 5 = 10
3	wtrPA- 20	High (10 μg)	IM	Days 0 and 28	0.5 mL	5 / 5 = 10
4	wtrPA- 5	Mid (2.5 μg)	IM	Days 0 and 28	0.5 mL	5 / 5 = 10
5	wtrPA- 1.25	Low (0.625μg)	IM	Days 0 and 28	0.5 mL	5 / 5 = 10
6	mrPA-20	High (10 μg)	IM	Day 0 and 28	0.5 mL	5 / 5 = 10
7	mrPA-5	Mid (2.5 μg)	IM	Day 0 and 28	0.5 mL	5 / 5 = 10
8	mrPA-1.25	Low (0.625μg)	IM	Days 0 and 28	0.5 mL	5 / 5 = 10
9	AVA	Human dose	IM	Days 0 and 28	0.5 mL	5 / 5 = 10
						Total = 45 / 45 = 90

^aAnimals vaccinated (0.5 mL per injection) with test or control article on Days 0 and 28.

^bEquivalent numbers per sex, 5 males and 5 females per group.