Skip to main content
. 2015 Jul 24;10(7):e0132950. doi: 10.1371/journal.pone.0132950

Fig 3. The three desirable estrogenic attributes of the NPF are not retained in one fraction after CCC fractionation.

Fig 3

To establish retention of the desirable estrogenic attributes of the NPF, promoter reporter and proliferation studies were conducted using E2 (positive control), the NPF, F1, F2, and F3. Promoter reporter studies in HEK293 cells transfected with ERE.vit2.luc and either pSG5-hERα (A&C) or pSG5-hERβ (B&D) were conducted in agonist (A&B) and antagonist (C&D) mode. Proliferation studies using MCF-7 BUS cells were conducted in agonist (E) and antagonist (F) mode. Statistical analysis was done using One-way ANOVA with Dunnett’s post-test comparing all columns to either solvent control (black bar) (*, P<0.05; **, P<0.01;***, P<0.001) or E2 (grey bar) (#, P<0.05; ##, P<0.01; ###, P<0.001). The dotted lines through the bars represent the values for solvent control and/or E2 used for statistical analysis.