Table 1. Quantification of major and some minor phenolic compounds in SM6Met, PF and NPF as determined by qHPLC.
| SM6Met | PF | NPF | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| # | Polyphenol | g/100g a | Concentration factor b | Yield (%) c | g/100g | Concentration factor | Yield (%) | g/100g | Concentration factor | Yield(%) |
| 4 d | Mangiferin | 1.90 | 1.00 | 100 | 0.09 | 0.05 | 3.22 | 5.56 | 2.93 | 82.35 |
| 5 | Isomangiferin | 0.65 | 1.00 | 100 | 0.05 | 0.08 | 5.22 | 1.88 | 2.91 | 81.39 |
| 6 | Vicenin-2 (apigenin-6,8-di-C-β-D-glucoside) | 0.19 | 1.00 | 100 | 0.10 | 0.53 | 35.74 | 0.37 | 1.95 | 54.80 |
| 10 | Scolymoside (luteolin-7-O-rutinoside) | 2.87 | 1.00 | 100 | 0.24 | 0.08 | 5.68 | 8.25 | 2.88 | 80.89 |
| 13 | Luteolin | 0.04 | 1.00 | 100 | Nd e | Nd | 0.00 | 0.11 | 2.63 | 77.39 |
| 1 | Iriflophenone-3-C-β-D-glucoside-4-O-β-D-glucoside | 0.88 | 1.00 | 100 | 1.02 | 1.16 | 78.70 | 0.60 | 0.68 | 19.19 |
| 3 | Iriflophenone-3-C-β-D-glucoside | 0.58 | 1.00 | 100 | 0.20 | 0.34 | 23.41 | 1.36 | 2.34 | 65.98 |
| 8 | 3-Hydroxy-phloretin-3',5'-di-C-hexoside | 1.16 | 1.00 | 100 | 0.38 | 0.32 | 22.24 | 2.79 | 2.41 | 67.68 |
| 11 | Phloretin-3',5'-di-C-β-D-glucoside | 1.77 | 1.00 | 100 | 0.35 | 0.20 | 13.43 | 4.79 | 2.70 | 76.15 |
| 9 | Eriocitrin (eriodictyol-7-O-rutinoside) | 0.85 | 1.00 | 100 | 0.19 | 0.22 | 15.18 | 2.13 | 2.52 | 70.52 |
| 12 | Hesperidin (hesperetin-7-O-rutinoside) | 2.05 | 1.00 | 100 | 0.37 | 0.18 | 12.26 | 5.36 | 2.61 | 73.58 |
| 7 | p-Coumaric acid | co-el f | 1.00 | co-el | Nd | Nd | 0.00 | co-el | co-el | co-el |
| 2 | Protocatechuic acid | 0.11 | 1.00 | 100 | Nd | Nd | 0.00 | 0.35 | 3.07 | 89.54 |
ag/100 g refers to the amount of the compound present (g) in 100 g extract/fraction.
bConcentration factor refers to the amount of a compound in an extract or fraction divided by the amount of the compound in SM6Met. For example, for mangiferin in the PF the concentration factor is (0.09 g/1.90 g) = 0.05.
cYield was calculated relative to the initial concentration of a compound in SM6Met, which was set to 100%. For example, for mangiferin in the PF, the yield is (0.061 g/1.90 g) x 100 = 3.22%, where 0.061 g (0.09 g x 67.9 g/100 g) refers to the total amount of mangiferin present in the PF as only 67.9 g of the PF is obtained during fractionation of 100 g SM6Met (as illustrated in S1 Fig).
dRefers to peak number in qHPLC chromatogram in Fig 1.
eNd—polyphenols were not detected due to absence or trace amounts.
fco-el—Co-elution refers to the fact that p-coumaric acid co-elutes with 3-hydroxyphloretin-3',5'-di-C-hexoside.