Fig 2. Hypoxia increases miR-21 and reduces PTEN levels in HPASMC in vitro and in the mouse lung in vivo.

HPASMC were exposed to normoxic or hypoxic conditions for 24–72 hours (A,C,D) and mice were exposed to normoxia (21% O₂) or hypoxia (10% O₂) for 3 weeks (B,E). Large and small mRNA species were isolated from HPASMC and mouse lung lysates, and miR-21 and PTEN levels were quantified. A representative immunoblot is shown in panel D. Each bar represents mean ± SEM miR-21 or PTEN levels. *P< 0.05 vs Normoxia-24 Hr, ^#P< 0.05 vs Normoxia-72 Hr, ⁺P< 0.05 vs Normoxia (Lung), ***P< 0.0001 vs Normoxia (HPASMC). A. (n = 6, 24 hr; n = 10, 72 hr); B. (n = 3); C,D. (n = 6); E. (n = 8).