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. 2015 Jul 24;10(7):e0133391. doi: 10.1371/journal.pone.0133391

Fig 6. Rosiglitazone does not attenuate HPASMC proliferation in the absence of PTEN.

Fig 6

HPASMC were transfected with PTEN small interfering RNA constructs. (A) PTEN mRNA and (B) PTEN protein levels were examined after transfection with PTEN siRNA. A representative immunoblot can be found in panel B. (C) HPASMC proliferation was assessed in PTEN-depleted HPASMC. In selected groups, RSG (10 μM) was added during the last 24 hours of the 72-hour incubation period prior to conducting the MTT proliferation assay. Each bar represents mean ± SEM HPASMC (A) PTEN mRNA, (B) PTEN protein, or (C) proliferation. *P< 0.05 vs Scrambled, ***P< 0.0001 vs Scrambled, +P< 0.01 vs Scrambled-Vehicle, #P< 0.05 vs Scrambled-RSG (n = 3–4).