Table 2. Isolation time (days) of cuticles of four citrus cultivars using leaf disks of different sizes and different concentrations of pectinase and cellulase.

Concentration	Cultivar	Large (10mm)	Medium (7mm)	Small (4mm)
4% pectinase + 0.4% cellulase	Lemon	8.33 ± 0.29 b	5.17 ± 0.29 b	2.00 ± 0.00 b
	Grapefruit	8.67 ± 1.61 b	5.50 ± 0.50 b	2.00 ± 0.00 b
	Valencia	15.50 ± 0.00 a	11.00 ± 2.50 a	3.00 ± 0.29 a
	Orange	9.83 ± 1.15 b	6.50 ± 0.00 b	2.17 ± 0.00 b
2% pectinase + 0.2% cellulase	Lemon	8.17 ± 0.76 b	5.00 ± 0.50 c	1.67 ± 0.58 b
	Grapefruit	8.33 ± 0.29 b	6.67 ± 0.76 b	2.33 ± 0.29 ab
	Valencia	15.67 ± 0.29 a	15.00 ± 0.50 a	2.67 ± 0.29 a
	Orange	9.33 ± 3.62 b	6.50 ± 1.00 b	2.33 ± 0.29 ab
1% pectinase + 0.1% cellulase	Lemon	NI	5.83 ± 0.29 c	2.17 ± 0.29 b
	Grapefruit	NI	6.83 ± 0.58 b	2.50 ± 0.00 b
	Valencia	NI	15.50 ± 0.00 a	3.50 ± 0.00 a
	Orange	NI	7.00 ± 0.50 b	2.33 ± 0.29 b

All data were analyzed by Duncan’s multiple range test using SAS software package. Values denoted by the same letter (a or b) are not significantly different within the same treatment at p≤ 0.05 level. NI indicates that cuticles could not be isolated from 10 mm disks in weak enzyme solution.