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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Cell Signal. 2015 May 28;27(9):1751–1762. doi: 10.1016/j.cellsig.2015.05.015

Figure 2. LPA2 is functionally upregulated and it maintains its functionality after irradiation as measured by Ca2+ assay.

Figure 2

Panel A. IEC-6 cells irradiated with 15 Gy were treated with an LPA2 specific agonist 24 h later to elicit a Ca2+ response. Data represents the mean of two experiments ± SD performed in triplicate. Statistical analysis of the data between the groups was performed by a Student’s t-test. *P < 0.05. Panel B. The LPA2 receptor accelerates the repair kinetics of DNA damage. Kinetics of γH2AX formation and resolution in irradiated IEC-6 cells treated with 10μM LPA for 15 minutes prior to irradiation. γH2AX was measured at the indicated time points by flow cytometry. Panel C. Pharmacological inhibition of LPA2 dose-dependently abrogates the effect of LPA. Cells were treated with 0.2μM and 1μM LPA2 antagonist Amgen compound 35 (ANT) 30 min prior to the addition of 10 μM LPA or vehicle 15 min before irradiation with 15 Gy. γH2AX was measured 6 h after irradiation with flow cytometry. Data shown are representative of three experiments. Statistical analysis of the data between the groups was performed by a Student’s t-test. *P < 0.05.