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. 2015 Jul 25;15:550. doi: 10.1186/s12885-015-1550-0

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© Subhash et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — GTSE1 expression in cisplatin treated AZ521 cells (a) AZ521 cells were treated with varying concentrations of cisplatin. The whole cell lysates were extracted post 24 h of treatment and GTSE expression was detected by western blotting. GAPDH served as loading control (b) GTSE1 sub cellular localization: AZ521 cells were treated with cisplatin for various time intervals and subcellular fractions were extracted and blotted for GTSE1 expression. Lamin and tubulin served as loading controls for nuclear and cytoplasmic fractions respectively