Fig. 3.

Transgenic E. nieschulzi oocyst expressing DHFR/TS-YFP (green) and EnOWP2-promoter controlled mCherry (red); construct 1. a: Freshly harvested unsporulated oocyst 0.5 h post isolation with nuclear and cytoplasmatic YFP fluorescence signal. b: Sporulating oocyst at 19 h post isolation with YFP and mCherry signals in the condensed cytoplasm. c: Sporulating oocyst at 33 h post isolation showing the mitotic division in the formed sporoblasts. YFP and mCherry are localised to the cytoplasm. d: Sporulated oocyst at 3 d post isolation. YFP signal are localised to cytoplasm and nucleus of the sporozoites, mCherry signals are localised to sporozoite cytoplasm and the sporocyst residue (granular signal). Scale bar: 5 μm. TL: transmitted light; UV: UV auto fluorescence of the oocyst wall; YFP: YFP fluorescence; mCherry: mCherry fluorescence; MCF: multichannel fluorescence, overlay of all fluorescent signals in the row