Table 1.
Proportion of transgenic oocysts (at least one fluorochrome is visibly expressed) in the single passages of parasites transformed with the pDLY-EnOWP2-promoter_mC, pDLY_EnOWP2_mC, pDLY_EnOWP2Exon1_mC, and pDLY_EnOWP6_mC construct and selected by pyrimethamine
| Construct | P | Part of transformed oocystsa (trOoc/total) | Part of mcherry + oocysts in trOoc* | Part of yfp + oocysts in trOoc | Proportion of YFP expressing sporocyst per transformed sporulated oocystb | |||
|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | |||||
| construct 1 | P0 | n.d. | n.d. | n.d. | n.d | n.d. | n.d. | n.d. |
| EnOWP2 promoter mCherry | P1 | 50 % (26/52) | 100 % (26) | 100 % (26) | 15 % | 35 % | 4 % | 46 % |
| construct 2 | P0 | 33 % (41/123) | 95 % (39) | 100 % (29) | 12 % | 24 % | 7 % | 56 % |
| EnOWP2-mCherry | P1 | 42 % (75/180) | 96 % (72) | 100 % (75) | 11 % | 37 % | 5 % | 47 % |
| P2 | 56 % (74/131) | 100 % (74) | 100 % (74) | 16 % | 33 % | 5 % | 45 % | |
| P3 | 64 %(102/159) | 100 % (102) | 97 % (99) | 20 % | 27 % | 6 % | 43 % | |
| construct 3 | P0 | n.d. | n.d. | n.d. | n.d | n.d. | n.d. | n.d. |
| EnOWP2-Exon1_ mCherry | ||||||||
| P1 | 26 % (45/170) | 67 % (30) | 100 % (45) | 4 % | 40 % | 4 % | 51 % | |
| construct 4 | P0 | 36 % (45/123) | 42 % (high) | 100 % (45) | 9 % | 29 % | 7 % | 55 % |
| EnOWP6 -mCherry (construct 4 | ||||||||
| P1 | 21 % (25/120) | 96 % (24) (46 % high 54 % low) | 100 % (25) | 16 % (4) mC low | 16 % (4) mC low | 8 % (2) mC low | 60 % (15) mC high: 11 mC low: 3 no mC: 1) | |
aat least one fluorochrome microscopically visible *
brounded, n.d. not determined