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. Author manuscript; available in PMC: 2015 Oct 13.
Published in final edited form as: Nat Commun. 2015 Apr 13;6:6768. doi: 10.1038/ncomms7768

Figure 3. Neuronal HttQ91 and glial HttQ25 aggregates associate with cytoplasmic markers.

Figure 3

(a–c) Co-localization of HttQ91 or HttQ25 aggregates with the endogenous cytoplasmic markers Hsp70/Hsc70, Hsp90, and ubiquitin. Single 1.0μm optical Z-slices from the DA1 glomerulus of 7 day-old adult flies expressing HttQ91-mCherry in DA1 ORNs and HttQ25-YFP in glia and immunostained with antibodies against Hsp70/Hsc70 (a), Hsp90 (b), and ubiquitin (c). Unfilled arrowheads indicate HttQ91 aggregates that lack associated HttQ25 signal, and filled arrows indicate induced HttQ25 aggregates in glia. Scale bars, 5μm. (d) Quantification of the % co-localization of each aggregate subtype with the endogenous cytoplasmic markers (n ≥ 4, ± s.e.m., * p < 0.05, ** p < 0.01, *** p < 0.001, one-way ANOVA followed by Tukey’s post hoc test).