Figure 4.

Effects of PrP^C (1-ORPD) on cell cycle distribution in gastric cancer cells. (A) Cell cycle distribution in transfected cells. The cells were detergent extracted, stained with propidium iodide, and analysed by flow cytometry. The PI were calculated: PI = (S+G2)/(G1+S+G2). (B) Cell cycle synchronization. Effect of PrP^C (1-OPRD) on cell cycle by arresting cells at G1/S boundary by adding thymidine (2 mM) at 0 hrs. Four hours after releasing, the cells began entering into S-phase. The average releasing rate of cells from G1- to S-phase in SGC7901/PrP^C (1-OPRD) was higher than that of SGC7901/PrP^C, even higher than that of vector control cells. Eight hours later after releasing, most cells had gone into G2-phase. By 16 hrs, cells entered a new cell cycle.