Table 2.

PCR primers and reaction parameters that were used for cyclin and CDK family genes

Gene	Primers	Denaturation	Annealing	Extension	Cycles
CyclinD1	F:5′- GGAGCTGCTCCTGGTGAACA -3′	94°C; 45′	59°C; 30′	72°C; 45′	35
	R:5′- TGTTGGGGCTCCTCAGGTTCA -3′
CyclinD2	F:5′- CCAGCAGGATGAGGAAGTGA -3′	94°C; 45′	59°C; 30′	72°C; 45′	35
	R:5′- GACGGTACTGCTGCAGGCTATT -3′
CyclinD3	F:5′- CATCCATGATCGCCACG -3′	94°C; 45′	59°C; 30′	72°C; 45′	35
	R:5′- GGAGCTGGTCTGAGAGGCT -3′
CDK4	F:5′- GCATCCCAATGTTGTCCG-3′	94°C; 45′	50°C; 30′	72°C; 45′	26
	R:5′- GGCAGCCCAATCAGGTCA-3′
b-actin	F:5′-ATG ATA TCG CCG CGC TCG TC-3′	94°C; 45′	50°C; 30′	72°C; 45′	23
	R:5′-CGC TCG GTG AGG ATC TTC A-3′