Figure 5.

Cx43 localization in alveolar septa during inflammation. Images of alveoli (A, B, C) and bronchi (D, E, F) from mice subjected (B, E, C, F) or not (A, D) to a 24 hrs IT LPS instillation are shown. An abundant staining for Cx43 in alveolar septa was observed after LPS instillation (B, arrows) as compared to untreated animals (A). The specificity of the staining was evaluated by pre-absorption of the antibody with its corresponding immunogenic peptide; under these conditions, Cx43 was no longer observed in alveolar septa and in smooth muscle cells of inflamed airways (C, F). The images are representative from six untreated and eight LPS-treated mice. The Cx43 staining is in green. Sections were counter-stained with Evans blue, in red. Scale bar represents 15 μm.