Figure 7.
Treatment of murine cell lines with 43Gap26 blocking peptide reduced neutrophil adhesion. Lucifer Yellow injections on pneumocyte (MLE12) and endothelial (bEnd.3) cell monolayers are shown in (A) and (C), respectively. Images are representative from three individual experiments. Both cells lines showed a decreased coupling when treated with 43Gap26 as compared to the control peptide condition. Neutrophil adhesion on MLE12 and bEnd.3 cell monolayers is presented in (B) and (D), respectively. Data are shown as percent of the number of neutrophils (PMN) that adhered under control conditions. Neutrophils adhered 14% (P < 0.05) and 40% (P < 0.01) less on MLE12 and bEnd.3, respectively, as compared to conditions with control peptide. Data are representative of four to six experiments. *Indicates P < 0.05; ** indicates P < 0.01.