Figure 5.

Identification of GC elements within Prm1 regions from –6848 to –6648 and –6258 to –6123. (A) and (B): Schematic of the Prm1 region from –6848 to –5895 and the relative positions of GC elements containing putative overlapping WT1/Egr1/Sp1 binding sites, where the 5′ nucleotide is indicated and the star symbol signifies mutated elements. Recombinant pGL3Basic encoding: (A) Prm1D (–6848), Prm1D^GC*^(–6717), Prm1E (–6648), Prm1I (–6258), Prm1I^GC*^(–6206) or Prm1J (–6123); or (B) Prm1D, Prm1D^GC*^(–6717), Prm1D^GC*^(–6206), Prm1D^GC*^{(–6717,–6206)} or Prm1E were co-transfected with pRL-TK into HEL 92.1.7 cells. Luciferase activity was expressed as mean firefly relative to renilla luciferase activity (RLU ± S.E.M.; n= 9).