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. 2008 Sep 16;13(11-12):4608–4622. doi: 10.1111/j.1582-4934.2008.00499.x

Figure 5.

Figure 5

MSP58 suppression modulated migration and invasion in U251 cells. (A) Effect of MSP58 depletion on cell migration. Monolayer of U251, U251-H1 neo, U251-NC and U251-S cells were mechanically wounded with a pipette tip. Repair of the lesion by cell migration was photographed 24 h later. (B) Quantification of cell migration. The distance of cell migration was calculated as described in “Materials and Methods”. Data were obtained from three independent experiments and presented as mean ± SD. (C) Representative photographs of wound-healing assay at time 0 and 24 hours in U251-NC and U251-Smix cells. (D) Quantification of U251-Smix cell migration compared to U251-NC. (E) MSP58 shRNA diminished cell invasion of U251 cells. Each of the indicated U251 cell types was assayed for cell invasion using transwell tissue culture dishes (8 μm pore size). (F) The average cell counts of invading cells from 6 high power fields (HPFs). Values were the mean ± SD obtained from three independent experiments. *P < 0.05 versus U251, U251-H1 neo, and U251-NC control cells.