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Characterization of 2′-SCF3 modified RNA. Anion-exchange HPLC traces (top) of: A) 8 nt RNA, B) 21 nt RNA, and C) 21 nt RNA, and the corresponding LC-ESI mass spectra (bottom). HPLC conditions: Dionex DNAPac column (4×250 mm), 80 °C, 1 mL min−1, 0–60 % buffer B in 45 min; buffer A: Tris-HCl (25 mM), urea (6 M), pH 8.0; buffer B: Tris-HCl (25 mM), urea (6 M), NaClO4 (0.5 M), pH 8.0. For LC-ESI MS conditions, see the Supporting Information.
