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Thermal stabilities of unmodified and 2′-SCF3 modified oligoribonucleotides. UV-melting profiles of A) self-complementary 8 nt RNA, B) 15 nt RNA hairpin, C) 15 nt RNA hairpin, and D) 12 nt RNA hairpin. Conditions: cRNA=8 μM; 10 mM Na2HPO4, 150 mM NaCl, pH 7.0. Nucleotide abbreviations in bold indicate the positions for 2′-SCF3 modification.
