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. 2015 Jun 12;21(29):10400–10407. doi: 10.1002/chem.201500415

Figure 3.

Figure 3

19F and 1H, 1H ECOSY NMR spectra of single-stranded RNAs: A) 5′-UU(2′-SCF3-A)GCG, and B) 5′-UU(2′-SCF3-G)UUU. For the 2′-SCF3 moiety, the 3-bond scalar coupling constants of H1′ and H2′ (3JH1′-H2′) were determined to be 9.7 and 8.0 Hz, respectively. These account for 96 and 80 % C2′-endo (South) populations in C2′/C3′-endo equilibria.[35,36] Note that for pyrimidine nucleosides the C2′-endo (South) population was 100 %.[10,11] Conditions: cRNA=0.3 mM; 25 mM sodium cacodylate, pH 7.0, 298 K.