Table 3.

Regulatory elements available for the C. reinhardtii chloroplast. Chloroplast functional parts are classified as transcriptional leaders (promoter and 5′UTR), chloroplast-targeted peptides, selectable markers and destination sequences for homologous recombination

Functional element	Name	Properties	Source
Transcriptional leaders	AtpA, psbD	Constitutive expression	Barnes et al. (2005)
	psaA-exon1	Strong expression	Michelet et al. (2011)
Chloroplast-targeted peptides	psaD	35 aa N-terminal	Fischer and Rochaix (2001)
	rbcS2, FD	32 aa N-terminal	León et al. (2007)
Selectable marker	rrnS and rrnL point mutations	Spectomycin and erythromycin resistance	Kindle et al. (1991)
	arg9	Arginine complementation	Remacle et al. (2009)
	aadA	Spectinomycin resistance	Goldschmidt-Clermont (1991)
	aphA6	Resistance kanamycin	Bateman and Purton (2000)
	GFP	Fluorescent reporter	Franklin et al. (2002)
	gusA	β-Glucuronidase activity	Sakamoto et al. (1993)
	atpB	5-Fluorodeoxyuridine resistance	Kindle et al. (1991)
	coda	Cytosine deaminase sensitivity to 5-fluorocytosine	Young and Purton (2014)
Destination sequences for homologous recombination	p322	psbA and 16S rRNA	Manuell et al. (2007), Barnes et al. (2005)
	patpint-cg11 (atpB-int)	Inverted repeat and atpB 3′UTR	Nickelsen et al. (1994)
	pLM7 (IR-int)	psbA and 5S/23S	Michelet et al. (2011)
	p72B	psbH and psbN	Bateman and Purton (2000)
	p71	tscA and inverted repeat	Kindle et al. (1991)