Skip to main content
. 2015 Jun 15;16(7):791–802. doi: 10.15252/embr.201540538

Figure 4.

Figure 4

DAZL acts as a translational enhancer of Tet1
  1. qRT–PCR results for genes involved in DNA methylation (Dnmt3b) and hydroxymethylation (Tet1 and Tet2), primed pluripotency (Gata6) and naïve pluripotency (Rex1, Prdm14, Tfcp2 l1 and Dazl) upon Dazl overexpression at d1 and d6 after treatment of ESCs with doxycycline.
  2. Mass spectrometric quantification of global 5hmC levels upon overexpression of Dazl in ESCs at d0, d1, d2, and d6.
  3. qRT–PCR results for Tet1 in both native as well as UV-cross-linked RNA-IP experiments in Dazl-GFP mESCs. Antibodies against GFP and V5 were used to immunoprecipitate Dazl, and normal mouse IgG was used as a negative control.
  4. Western blot for TET1 protein levels upon overexpression of Dazl in ESCs at d0, d1, d2, and d6. Relative enrichment of TET1 in different samples was quantified using ImageJ on Western blots of 3 biological replicates.
  5. Western blot for TET1 protein levels in Dazl+/+ and Dazl−/− ESCs upon conversion to 2i culture conditions. Relative enrichment of TET1 in different samples was quantified using ImageJ.

Data information: P-values were calculated using Student's t-test. *P < 0.05, **P < 0.001. Error bars indicate s.d. values of > 3 biological replicates.