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. 2015 Jun 13;16(7):813–823. doi: 10.15252/embr.201540137

Figure 5.

Figure 5

A functionally important hydrophilic patch on Ups1
  1. Location of the hydrophilic patch in the Ups1-Mdm35-PA structure. A close-up view of the electrostatic potential surface of the patch is shown on the right.
  2. Yeast (Ups1Δ) growth assay complemented with wild-type (WT) and mutant Ups1 containing mutations in the hydrophilic patch. Ups1-3E: Ups1 mutant containing H33E, K58E, and K61E mutations; Ups1-4E: Ups1 mutant containing H33E, K58E, K61E, and K148E mutations; Ups1-5E: Ups1 mutant containing H33E, K58E, K61E, K148E, and K155E mutations; and Ups1-5A: Ups1 mutant containing H33A, K58A, K61A, K148A, and K155A mutations.
  3. Mitochondrial morphology of the yeast cells (Ups1Δ) complemented by wild-type and mutant Ups1 was examined using confocal microscopy. Left: differential interference contrast (DIC); right: fluorescence (Su9-GFP). Scale bars: 1 μm.
  4. Quantitation of the results of (C). Values are the means ± SD (three independent experiments of (C) were performed and quantified).