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. 2015 Apr 21;145(4):485–497. doi: 10.1111/imm.12463

Figure 4.

Figure 4

Reconstitution of microRNA-155 (miR-155) in natural killer (NK) cells enhances interferon-γ (IFN-γ) production by inhibiting Tim-3/T-bet. (a) Purified CD3− CD56+ NK cells from hepatitis C virus (HCV) infected subjects were transfected by miR-155 mimic and negative control with a Lonza Human NK Nucelofector Kit and Nucleofector I Device, followed by real-time RT-PCR assay for miR-155 levels to determine the transfection efficiency. (b) Purified CD3− CD56+ NK cells from HCV subjects were transfected by miR-155 mimic and negative control and incubated in the presence of interleukin-2 (IL-2) for 48 hr, followed by flow cytometric analysis for intracellular IFN-γ production. Representative dot plots and summary data from three independent experiments are shown. (c) NK92 cells were transfected by miR-155 mimic and negative control and incubated with HCV+ Huh-7 cells in the presence of IL-2 for 48 hr, followed by flow cytometric analysis for Tim-3, T-bet, and IFN-γ expression. Representative dot plots and summary data from three independent experiments are shown. *P < 0·05.