Figure 2.
Intercellular adhesion molecule 1 (ICAM1) expression is regulated by microRNA 214 (miR-214) in Chlamydia muridarum-infected murine genital cells. Genital tract tissue from naive C57BL/6 (wild-type; WT) mice was excised and cultured ex vivo. Cells were seeded at a density of 2·5 × 105 and transfected with miR-214 inhibitor, mimic, or scrambled miRs (control). Untransfected genital cells were infected with multiplicity of infection 1 of C. muridarum elementary bodies (resuspended in medium) or incubated with medium alone (mock). ICAM1 expression was assessed by real-time PCR at 24 hr post challenge and expressed as relative value to untransfected mock group. *P < 0·05, **P < 0·01 (Student's t-test) compared with untransfected mock group. Data are representative of three individual experiments.