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The presence of stably-transfected OE33 (OE33/DKK3 +Endo) increased endothelial tube formation similar to the tube formation seen after adding VEGF to endothelial cells (Endo +VEGF) and to OE33/Vector (OE33/Vector +Endo +VEGF). Endothelial, native OE33, and vector controls showed minimal tube formation. OE33 and endothelial cells were labeled green and red respectively. Original magnifications were 200x.
