sox10 transgenic constructs and expression in zebrafish.
(A) Schematic representation of transgenic strategy used to generate NC:NfsB-mCherry and NC:mCherry lines. (B) Confocal image of NC:NfsB-mCherry fish at 19 hpf from a lateral view (anterior to left). The mCherry positive cells are found in cranial and trunk neural crest (box enlarged in inset). (C) Ventral view of the anterior region (indicated by red box on illustration) of NC:mCherry; kdrl:GFP embryo at 108 hpf (anterior to left). The mCherry positive cells are observed in craniofacial structures, as well as the heart (arrowhead) and around the ventral aorta (arrowhead). bh, basihyal; bsr3, branchiostegal ray 3; chb, ceratohyal; d, dentea; mx, maxilla; Mk, Meckel’s cartilage; op, opericle; pq, palatoquadrate. Scale bars = 100 µm.