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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: J Cell Biochem. 2015 Sep;116(9):2098–2108. doi: 10.1002/jcb.25167

Fig. 2. IL-11 regulates expression of osteolytic genes PTHrP and RANKL in PC3 cells.

Fig. 2

(A) Effect of TGFβ1 treatment on osteolytic gene expression in PC3-L cells. Relative MRNA expression of PTHrP and RANKL was determined using qPCR in PC3-L cells treated with DMSO-control or TGFβ1. (B) Secretion of IL-11 protein measured by testing a sample of the culture medium using ELISA following IL-11 knockdown using siRNA (NS siRNA: non-silencing control siRNA). (C) Expression of IL-11, PTHrP and RANKL mRNA following IL-11 knockdown in PC3-H cells compared to controls. Data in panels A and C were normalized to GAPDH and were statistically analyzed using the Student’s t-test with Welch’s correction: not significant (ns), * p<0.05, ** p<0.01.