Table 2.
LC-ESI/MS of the oligosaccharides obtained by digestion of the non-acid fractions N-III and N-IV from moose I small intestine with Rhodococcus endoglycoceramidase II. Summary of MS2 data
| Fraction | [M-H+]−
ion |
RT (min) | C ions (m/z) |
0,2A ions (m/z)a |
Carbohydrate sequence | Tentative oligosaccharide |
|---|---|---|---|---|---|---|
| N-III | m/z 503 | 20.0/21.3 | C1 179; C2 341 | 0,2A2 281 | Hex-4Hex-4Hex | Galα4Galβ4Glc |
| m/z 706 | 22.3 | C1 220; C2 382; C3 544 | 0,2A3 484 | HexNAc-Hex-4Hex-4Hex | GalNAcβ3Galα4Galβ4Glc | |
| m/z 487 | 25.2 | C1 163; C2 325 | – | Fuc-Hex-4Hex | Fucα2Galβ4Glc | |
| N-IV | m/z 503 | 20.2 | C1 179; C2 341 | 0,2A2 281 | Hex-4Hex-4Hex | Galα4Galβ4Glc |
| m/z 706 | 21.2/22.4 | C1 220; C2 382; C3 544 | 0,2A3 484 | HexNAc-Hex-4Hex-4Hex | GalNAcβ3Galα4Galβ4Glc | |
| m/z 544 | 25.9 | C1 at m/z 220, C2 at m/z 382 | – | HexNAc-Hex-4Hex | GlcNAcβ3Galβ4Glc | |
| m/z 706 | 28.9 | C2 382; C3 at m/z 544 | – | Hex-HexNAc-Hex-4Hex | Galβ3GlcNAcβ3Galβ4Glc | |
| m/z 706 | 29.2 | C2 382; C3 at m/z 544 | 0,2A2 281 | Hex-4HexNAc-Hex-4Hex | Galβ4GlcNAcβ3Galβ4Glc |
aThe 0,2A ions derived from the lactose unit at the reducing end are not given