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Real-time PCR analysis of expression of several virulence-factor genes. MRSA was cultured and treated with subminimal inhibitory concentration (1–4 mg/mL) of A. princeps extract, and real-time PCR analysis was then performed as described in Section 2. Expression of mecA, sea, and agrA was significantly inhibited at concentration higher than 1 mg/mL. Each value is expressed as a mean ± standard deviation. Significance was determined at * P < 0.05 when compared with the control.
