Table 3.

Relative quantification and fold expression of differentially expressed genes in powdery mildew resistant “Natchez” (NAT) versus susceptible “Carolina Beauty” (CAB) cultivars.

Primer	Query name	NAT cDNA Ct (mean)	CAB cDNA Ct (mean)	Fold diff. (2-Nat-CabCt)∗	Fold diff. (2-Cab-NatCt)∗∗	Fold diff. (2-Nat-CabCt)∗	Fold diff. (2-Cab-NatCt)∗∗	Fold diff. (2-Nat-CabCt)∗	Fold diff. (2-Cab-NatCt)∗∗	Mean fold
cab-h1	B.9985	27.8	26.78
cab-h8	B.8346	27.83	28.75
nat-h1	T.9454	29.40	29.30
cab-rt1	B.7344	32.18	30.94	—	1.16	—	4.47	—	2.20	2.61
cab-rt2	B.2282	30.19	29.92	—	0.59	—	2.28	—	1.13	1.33
cab-rt3	B.3011	31.50	33.31	—	0.14	—	0.54	—	0.27	0.32
cab-rt5	B.18652	29.02	26.48	—	2.87	—	11.00	—	5.43	6.43
cab-rt6	B.21408	35.94	37.44	—	0.17	—	0.67	—	0.33	0.39
cab-rt7	B.21856	35.16	33.60	—	1.45	—	5.58	—	2.75	3.26
cab-rt8	B.22624	33.80	28.64	—	17.63	—	67.65	—	0.17	28.48
cab-rt9	B.22625	29.36	26.84	—	2.83	—	10.85	—	5.35	6.34
cab-rt10	B.23059	32.30	34.69	—	0.09	—	0.36	—	0.18	0.21
nat-rt6	T.13188	32.75	37.73	0.20	—	0.05	—	0.11	—	0.12
nat-rt8	T.13192	28.34	29.43	4.23	—	1.10	—	2.23	—	2.52
nat-rt9	T.13486	32.08	29.40	0.21	—	0.05	—	0.11	—	0.12
nat-rt11	T.17520	37.91	28.78	1.79	—	0.47	—	0.95	—	1.07
nat-rt13	T.8775	28.14	27.46	1.27	—	0.33	—	0.67	—	0.75
nat-rt14	T.9193	29.82	31.05	4.76	—	1.24	—	2.51	—	2.84
nat-rt15	T.17643	30.33	28.44	0.55	—	0.14	—	0.27	—	0.32

Note. We calculated the fold increase in expression for the NAT primers by treating NAT as the treatment and housekeeping gene as the control and compared to CAB treatment (∗). For the CAB primers we used CAB as the treatment and housekeeping gene as the control and compared to NAT treatment (∗∗). Highlight in bold (cab-h1), italic (cab-h8), and bold italic (nat-h1) indicates that fold difference of expression was calculated based on individual housekeeping gene. Mean fold was calculated from three housekeeping genes.