Figure 1.

Flow cytometry and multipotency analysis. Flow cytometry dot plots representative for human adipose derived mesenchymal stem cells (hASCs) (a). Isolated cells are characterized by the presence of CD29, CD44, CD73b, CD90, and CD105. hASCs lack the CD45 surface markers, as well as the CD34hematopoietic lineage marker. Flow cytometry analysis determined the percentage of specific markers in total analyzed hASCs from patients in different age (b). Quantification of markers revealed no significant differences between groups. Representative images from trilineage assay of hASCs after 21 days of differentiation (c). Osteogenically differentiated cells in monolayer stained with Alizarin Red, chondrogenically differentiated cells using Safranin O, and adipogenically differentiated cells stained using Oil Red O. Multipotency test was performed on ASCs isolated from patients between 25 and 77 age ranges. Magnification ×100, scale bars: 500 μm. Results expressed as mean ± SD. ^∗ P value < 0.05.