Table 1.
Sequences of primers used in qPCR. PPARγ: peroxisome proliferator-activated receptor gamma; COL-1: collagen type I; OPN: osteopontin; BMP-2: bone morphogenetic protein 2; ALP: alkaline phosphatase; OCN: osteocalcin; p53: tumor suppressor p53; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; bp: base pair.
| Gene | Primer | Sequence 5′-3′ | Amplicon length (bp) | Accession number |
|---|---|---|---|---|
| PPARγ | F: | AGTCCTCACAGCTGTTTGCCAAGC | 125 | XM_011533844.1 |
| R: | GAGCGGGTGAAGACTCATGTCTGTC | |||
|
| ||||
| COL-1 | F: | GTGATGCTGGTCCTGTTGGT | 123 | NM_000088.3 |
| R: | CACCATCGTGAGCCTTCTCT | |||
|
| ||||
| OPN | F: | AAACGCCGACCAAGGTACAG | 213 | U20758.1 |
| R: | ATGCCTAGGAGGCAAAAGCAA | |||
|
| ||||
| BMP-2 | F: | ATGGATTCGTGGTGGAAGTG | 349 | KC294426.1 |
| R: | GTGGAGTTCAGATGATCAGC | |||
|
| ||||
| ALP | F: | CGCGCTTGTGCCTGGA | 185 | XM_006710546 |
| R: | CCTGCTTTATCCCTGGAGCC | |||
|
| ||||
| OCN | F: | ATGAGAGCCCTCACACTCCTC | 292 | NM_199173.4 |
| R: | CGTAGAAGCGCCGATAGGC | |||
|
| ||||
| p53 | F: | AGATAGCGATGGTCTGGC | 381 | NM_001126118.1 |
| R: | TTGGGCAGTGCTCGCTTAGT | |||
|
| ||||
| GAPDH | F: | CATGGCCTTCCGTGTTCCTA | 286 | NM_017008.4 |
| R: | CACCACCCTGTTGCTGTAGC | |||