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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Integr Biol (Camb). 2015 Apr;7(4):454–466. doi: 10.1039/c5ib00052a

Fig. 10. Analysis method for vertical anastomosis assay.

Fig. 10

(A) Stacks of 3μm-spaced images were collected using confocal microscopy. Every well represents an independent experiment. Three random non-overlapping locations within a well were used for averaging. (B) A custom Matlab® code was created to separate the red and green channels from the collected image stacks. The total intensity of an image (I) is tracked along z-direction to characterize the distribution of red and green structures. m is the linear index of the intensity matrix elements.