Fig. 5. Depth distribution of cell populations with time.
(A–B) Distributions of the fluorescence intensity in the depth measured from the position of the monolayer. Cells initially seeded on the surface of the gel (RFP+-HUVECs) (A); cells initially distributed within the bulk (GFP+-HUVECs) of the gels (B). BCs were seeded at a density of 1×106 cells/mL; MCs at 250 cells/mm2. Time-points of Day 1 (black), Day 3 (red), Day 5 (blue), and Day 7 (grey) are shown. (C) Comparison of intensity-weighted average depth of invasion in Mono Only, “MO” and Mono + Bulk (medium), “MB (medium)” configurations. (D) Comparison of intensity-weighted average depth of BCs in Bulk Only (medium), “BO (medium)” and Mono + Bulk (medium), “MB (medium)” configurations. Error bar: Standard Deviation. (ns: not significant; *: p < 0.05; **: p < 0.01; ***: p < 0.001)