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. Author manuscript; available in PMC: 2016 Apr 8.
Published in final edited form as: Oncogene. 2015 Jan 26;34(41):5288–5294. doi: 10.1038/onc.2014.455

Figure 1. Cotransin suppresses the expression of HER3.

Figure 1

A. BT474 cells were treated with the indicated concentrations of CT8 for 24 or 48 hours and cell lysates were assayed by western blotting as indicated. B. Several HER2-amplified cancer cells were treated with increasing concentrations of CT8 for 24 hours and cell lysates were assayed for HER3 expression by western blotting. C. HEK-293 cells were transiently transfected with a pcDNA-DEST40 vector containing the open reading frames of EGFR, HER2, HER3, or HER4, in-frame with a c-terminal V5 tag. Transfectants were treated with 500nM CT8 for 24 hours and subjected to western blot analysis using anti-V5 and anti-actin antibodies.