Table 1.
Semiquantitative analysis of inflammatory cells present in the skin of BALB/c mice from 3 to 96 hours after pVAX-EGFP vaccination followed by electroporation.
| Time after intradermal EP vaccination | Gr-1 | CD11b | CD68 | CD11c | CD4 | B220 | CD8 |
|---|---|---|---|---|---|---|---|
| 3 h | +++ | +++ | ++ | + | + | −/+ | − |
| 6 h | ++ | +++ | ++ | ++ | +++ | + | −/+ |
| 24 h | ++ | ++ | + | + | ++ | + | −/+ |
| 48 h | + | ++ | + | −/+ | + | ++ | − |
| 72 h | + | ++ | + | −/+ | + | ++ | − |
| 96 h | −/+ | ++ | + | −/+ | −/+ | ++ | − |
The expression of reactive cells was defined as absent (−), scarcely (−/+), moderately (+), frequently (++), or strongly (+++) present on cryostat sections stained with the antibodies.