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. 2015 Jul 27;8:40. doi: 10.3389/fnmol.2015.00040

Figure 2.

Figure 2

DNAJB6 depends on HPD motif to prevent Aβ42-GFP aggregation. (A) HEK293 cells were transfected with Ub-Aβ42-GFP with either DNAJA1, DNAJB1, DNAJB6 (Wt), DNAJB6 (H31Q) mutants at 1:3 ratio for 48 h. Cells were lysed, fractionated into T, S, P and Western blotting was performed (B) HEK293 cells were transfected with Ub-Aβ42-GFP with either FRTTO (control) or HSPA1a at 1:3 ratio for 48 h. Quantification of the pellet/soluble ratio of Aβ-GFP relative to FRTTO was depicted in the charts above the blots. Values represent mean ± SE of two independent experiments. *P < 0.05, ***P < 0.001, ns = non significant.