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. 2015 Jul 27;5:12529. doi: 10.1038/srep12529

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(A) Bright-field and time-lapse Raman images of the osteoblasts. The Raman images show cytochrome c (750 cm⁻¹) as green, HA (956 cm⁻¹) as red, β-carotene (1526 cm⁻¹) as magenta, and the CH₃ stretching mode (2940 cm⁻¹) as blue. (B) The individual time-lapse Raman images for β-carotene and HA. (C) The temporal changes in the averaged Raman spectra obtained from the same location in the osteoblasts. Each spectrum was averaged from 234 pixels (about 24 μm²).