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. 2015 Jul 27;5:12513. doi: 10.1038/srep12513

Figure 2. Identification of DptC/D/E enzymes functioning as a protein complex.

Figure 2

(A) Heterologous co-overexpression of dptCDE operon by labeling DptC with His-tag. (B) Detection of PT modification in the host strain of E. coli BL21 harboring heterologous dptCDE gene cluster. (C) SDS-PAGE analysis of the proteins co-purified with DptC. (D–F) Mass spectra of DptD, DptC, DptE, respectively, digested with trypsin by MALDI-TOF/TOF MS (left panels) and an analysis of MS/MS spectrum [M + H]+ acquired for the tryptic peptides with sequences listed (right panels); All the detected peptides by MALDI-TOF-TOF are underlined at each bottom.