Figure 3.

(A) SNORA62 conserved in the last introns of the FAU gene in S. domuncula, S. ficus and S. pagurorum. All essential snoRNA elements are conserved and a putative pseudouridylation (PU) guide site is designated; red = 100%, blue = 80% and yellow = 60% identity; (B) Target sites of SNORA62 are conserved in sponges and marked with psi; (C) Experimental verification of transcription of sponge SNORA62. Polyadenylated snoRNAs were amplified, cloned and sequenced; (D) Maximum likelihood (ML) phylogenetic tree of snoRNAs from representative species. Bootstrap values for ML are given above and MCMC below the line. The scale bar indicates the genetic distance of the branch lengths.