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. 2015 Jul 21;13(7):4505–4519. doi: 10.3390/md13074505

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Chromatographic analyses of the aqueous (Aq-MeOH), butanolic (BuOH-MeOH), and ethyl acetate (EtOAc-MeOH) phases of the methanolic extract. The chromatographs were obtained by reverse-phase analysis. The most effective inhibition of thrombin activity and the greatest increase in the coagulation time were observed mainly in the BuOH-MeOH and EtOAc-MeOH phases of the methanolic extract. These results suggest that the main compounds responsible for these effects can be considered inhibitors.