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. 2015 Jul 21;13(7):4505–4519. doi: 10.3390/md13074505

Figure 7.

Figure 7

(A) A chromogenic substrate for thrombin was used to measure thrombin inhibition by quercetin-3-O-rhamnoside (Qn) and quercetin-3-O-arabinoside (QAra). This assay was performed under the same conditions described elsewhere in this paper; (B) The effects of Qn and QAra on the coagulation time were measured using a turbidity test based on the average thrombin time. The bars represent the standard deviation, and * indicates significant differences in relation to the standard. All analyses were performed using analysis of variance (ANOVA, p < 0.05), and each bar represents n = 12. The spectroscopy results varied for all purified flavonoids over the course of the enzymatic experiment; (C) Enzyme kinetics of the compounds with the chromogenic substrate to see if there was interaction between them.