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. 2015 Jul 27;5:12425. doi: 10.1038/srep12425

Figure 6. OA became cytotoxic after lysosomal impairment.

Figure 6

(a) HaCaT keratinocytes were treated with DMSO (control) and CQ (25 μM) for 24 hours. (b) At indicative times cell survival was evaluated by MTT assay following calculation of AAU. The correlation between cell survival and AAU was evaluated as function of time T1, T2 and T3. (c) After treatment for 6 hours with DMSO (control), OA (20 μM) or CCCP (2 μM) in presence or absence of BAF (2 ηM) citrate synthase activity was presented as arbitrary units after normalization to control. Micrographs of treated cells at T1 with DMSO (control) and triterpenoids (20 μM) in presence or absence of BAF (2 ηM) treatment, following immunostaining for (d) the mitochondrial marker COXIV (green) and (e) p62 (red). At bottom panel, box-plots represented the fluorescence profile of multiple images. T1 = after treatment for 24 hours; T2 = 24 hours after T1; T3 = 48 h after T1. All results were obtained from at least three independent experiments and expressed as mean values ± standard error. Multiple statistical comparisons were calculated by ANOVA test, and the p-value for each pairwise group was determined by Dunnett’s T3 (high variance between groups) or Bonferroni (low variance between groups) post-hoc test. The analysis of correlation was done using Pearson’s coefficient (r). Significance difference (p < 0.05) was depicted by asterisk.