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(a) Insulin content in MIN6 cells measured by ELISA. (b) Insulin content in islet cells determined by ELISA. n = 3, *P < 0.05, **P < 0.01. (c) Insulin levels in MIN6 cells are visualized by immunostaining with insulin antibody. (d) Insulin levels in mouse islet cells are estimated by immunostaining with insulin antibody.
