Table 1.

Primer sequences, amplification and digestion conditions used in the study.

Polymorphisms	Primer sequences	PCR	Digestion	PCR and restrictionpatterns (bp)
CYP2E1 1053C > T	F: 5′-CCAGTCGAGTCTACATTGTCA-3′ R: 5′-TTCATTCTGTCTTCTAACTGG-3′	35 cycles: 95 °C for 1 min, 60 °C for 1 min, and 72 °C for 1 min	RsaI, overnight at 37 °C	PCR:410 CC: 360, 50 CT: 410, 360, 50 TT: 410
CYP2E1 7632T > A	F: 5′-TCGTCAGTTCCTGAAAGCAGG-3′ R: 5′-GAGCTCTGATGCAAGTATCGCA-3′	35 cycles: 94 °C for 30 s; 63 °C for 30 s; 72 °C for 1 min	DraI, 3–5h at 37 °C	PCR: 375 TT:375 TA:375, 249, 126 AA: 249, 126
NQO1 609 C > T	F: 5′-GAGACGCTAGCTCTGAACTGAT-3′ R: 5′-ATTTGAATTCGGGCGTCTGCTG-3′	30 cycles: 94 °C for 10 s; 57 °C for 20 s; 72 °C for 45 s	HinfI, overnight at 37 °C	PCR: 304 CC: 271 CT: 151, 120 TT: 271, 151, 120
MPO 463 G > A	F: 5′-CGGTATAGGCACACAATGGTGAG-3′ R: 5′-GCAATGGTTCAAGCGATTCTT-3′	35 cycles: 91 °C for 1 min; 59 °C for 1 min; 71 °C for 1 min	AciI, 3h at 37 °C	PCR: 350 GG: 169, 120, 61 GA: 289, 169, 120, 61 AA: 289, 61
GSTM1	F: 5′-GAACTCCCTGAAAAGCTAAAGC-3′ R: 5′-GTTGGGCTCAAATATACGGTG-3′	35 cycles: 94 °C for 2 min; 61 °C for 1 min; 72 °C for 2 min		PCR: 215
GSTT1	F: 5′-TTCCTTACTGGTCCTCACATCTC-3′ R: 5′-TCACCGGATCATGGCCAGCA-3′	35 cycles: 94 °C for 2 min; 61 °C for 1 min; 72 °CC for 2 min		PCR: 480
β-globin	F: 5′-CAACTTCATCCACGTTCACC-3′ R: 5′-GAAGAGCCAAGGACAGGTAC-3′	35 cycles: 94 °Cfor 2 min; 61 °C for 1 min; 72 °C for 2 min		PCR: 268