Skip to main content
. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2015 Mar 8;284(3):304–314. doi: 10.1016/j.taap.2015.02.022

Figure 3. OFC protects against hepatic inflammation.

Figure 3

Animals and exposures to high fat diet (HFD), arsenic (As), and the prebiotic oligofructose (OFC) were as described in the Methods. Panel A shows representative photomicrographs of immunohistochemical staining for F4/80 (200×) in which hepatic macrophages are stained brown. The left graph of panel B shows quantitation of F4/80 staining, which was determined by blinded counting of the number of F4/80 positive cells per 1000 hepatoctyes as described in Methods. The right graph of panel B shows real time RT-PCR for the pro-inflammatory gene TNFα, which was performed as described in Methods. Quantitative data are shown as means ± SEM (n = 6–10). a, p < 0.05 compared to tap water control; b, p < 0.05 compared to LFD; c, p < 0.05 compared to no OFC.