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. 2015 Jul 24;6:7857. doi: 10.1038/ncomms8857

Figure 3. Effect of ciliopathy genes on progenitor proliferation.

Figure 3

(ae) Co-labelling with anti-GFP and PH3 antibodies show proliferating, GFP+/PH3+ RG progenitors in VZ (arrow) and IPs in SVZ (arrowhead), respectively, in control cortices (a). (be) In contrast, knockdown of BUBR1 [BUB1B] (b), TMEM216 (c), IFT80 (d) and KIF7 (e) resulted in significantly reduced proliferation of RG progenitors and IPs. (fj) Co-labelling with anti-GFP, BrdU and Tbr2 antibodies indicates that knockdown of BUBR1 [BUB1B] (g), TMEM216 (h), IFT80 (i) and KIF7 (j) resulted in reduced percentage of Tbr2+ cells (Tbr2+/GFP+ (open arrowhead)) and mitotic progenitors (BrdU+/GFP+ (arrow), Tbr2+/BrdU+/GFP+ (filled arrowhead)). (k) Quantification of percentage of PH3+/GFP+ cells in control and BUBR1 [BUB1B], TMEM216, IFT80 and KIF7 groups. Data shown are mean±s.e.m. *P<0.05 (Student's t-test). (l) Quantification of percentage of BrdU+/GFP+, Tbr2+/GFP+ and Tbr2+/BrdU+/GFP+ cells in control and BUBR1 [BUB1B], TMEM216, IFT80 and KIF7 shRNA groups. Data shown are mean±s.e.m. *P<0.05 (Student's t-test). Number of brains per group=4. Scale bar, 10 μm (aj).

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