Figure 7.
Knockdown of MDC1 promotes breast cancer cell growth in null mice. (A) The effect of MDC1 depletion on the growth of transplanted tumor in null mice. 5-week-old female BALB/C-null mice were subcutaneously injected with 5×106 MCF-7 cells infected with lentiviruses harboring shCtrl or shMDC1. (B) Representative photographs of tumors in each group isolated from nude mice after 8 weeks injection. Tumor growth was monitored with the treatment of E2 pellets. (C) Tumors was measured weekly using a vernier caliper and the volume was calculated according to the formula π/6×length×width2. MDC1 depletion causes an increase in tumor volume. Each point represents the mean ± SD for different animal measurements (n=8). (D) Tumor was excised and weighed after 8 weeks. Average tumor weight of two groups was shown. Results represent the mean ± SD. P values were determined by student's t test. * P<0.05, ** P<0.01. (E) Schematic representation of MDC1 co-activator functions on ERα-induced transactivation and its role in suppression of breast cancer.